Simultaneous determination of serotonin,N-acetylserotonin and melatonin in the pineal gland of the juvenile golden hamster by high-performance liquid chromatography with electrochemical detection |
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| Affiliation: | 1. Radiation Signalling and Cancer Biology Section, Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400 085, India;2. Department of Radiation Oncology, Tata Memorial Hospital, Dr Ernest Borges Marg, Parel, Mumbai 400 012, India;1. Afyon Kocatepe University, School of Medicine, Department of Emergency Medicine, Afyonkarahisar, Turkey;2. Afyon Kocatepe University, School of Medicine, Department of Radiology, Afyonkarahisar, Turkey;3. İzmir Katip Celebi University, Atatürk Training and Research Hospital, Clinic of Emergency Medicine, Izmir, Turkey;4. Afyon Kocatepe University, School of Medicine, Department of Anatomy, Afyonkarahisar, Turkey;5. Afyon Kocatepe University, School of Medicine, Department of Biostatistics and Medical Informatics, Afyonkarahisar, Turkey;6. Bursa Yuksek Ihtisas Training and Research Hospital, Clinic of Emergency Medicine, Bursa, Turkey;7. Afyon Kocatepe University, School of Medicine, Department of Neurosurgery, Afyonkarahisar, Turkey |
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| Abstract: | A simple and simultaneous determination of melatonin and its precursors, serotonin (5-HT) and N-acetylserotonin, was achieved by reversed-phase high-performance liquid chromatography with electrochemical detection. The addition of an ion-paring agent, sodium 1-octanesulfonate, to the chromatographic mobile phase caused an increase of the retention time of 5-HT, and resulted in the successful simultaneous resolution of these three indoleamines. This method was used to quantitate these indoleamines in the pineal gland of juvenile golden hamsters. |
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