High-performance liquid chromatographic determination of tramadol in human plasma |
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| Affiliation: | 1. Department of Chemistry, Durban University of Technology, Durban, South Africa;2. Department of Chemistry, University of South Africa, Pretoria, South Africa;3. Molecular Sciences Institute, School of Chemistry, University of Witwatersrand, Johannesburg, South Africa |
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| Abstract: | Tramadol has been determined in human plasma samples using a sensitive high-performance liquid chromatographic method. The plasma samples were extracted with tert.-butylmethyl ether in one-step liquid-liquid extraction (recovery 86%) and analyses of the extracts were performed on reversed-phase silica gel using ion-pair chromatography (verapamil as an internal standard) and fluorescence detection. The method was applied to the determination of tramadol levels in twelve healthy volunteers after oral administration of 100 mg of tramadol in capsules of Protradon and Tramal. |
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