Determination of mivacurium in plasma by high-performance liquid chromatography |
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| Affiliation: | 1. MD Candidate, Harvard Medical School; Resident, Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital;2. Assistant Professor of Oral and Maxillofacial Surgery, Boston Children''s Hospital, Harvard School of Dental Medicine, Boston, MA, USA;3. Assistant Professor of Oral and Maxillofacial Surgery, Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Harvard School of Dental Medicine, Boston, MA, USA |
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| Abstract: | An assay has been developed and validated for the routine monitoring of mivacurium in plasma. It consists of liquid-liquid extraction with dichloromethane and high-performance liquid chromatography with fluorometric detection (excitation and emission wavelengths 220 nm and 320 nm, respectively). A Spherisorb C1 5 μm column and a mobile phase containing acetonitrile, KH2PO4 and methanol are used. At a flow-rate of 1 ml/min, a concentration gradient is applied. The detection limit is approximately 1 ng/ml in plasma. For the separation of stereoisomeres, the Spherisorb SCX 10 μm column and acetonitrile-Na2SO4 as a mobile phase can be used. The assay shows good linearity over the range 1–1000 ng/ml. The accuracy and precision allows the utilisation in clinical pharmacokinetic studies. |
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