Determination of midazolam and its metabolites in serum microsamples by high-performance liquid chromatography and its application to pharmacokinetics in rats |
| |
| Affiliation: | 1. Natural Products Institute, University of the West Indies, Mona, Kingston 7, Jamaica;2. University of Technology, Kingston 7, Jamaica;3. Biotech R&D Institute, 44 Lady Musgrave Road, Kingston 10, Jamaica;4. Scientific Research Council, Hope Gardens, Kingston 6, Jamaica |
| |
| Abstract: | A single-solvent extraction step high-performance liquid chromatographic method is described for quantitating midazolam and its two hydroxy metabolites in rat serum microsamples (50 μl). The separation used a 2 mm I.D. reversed-phase Symmetry C18 column with an isocratic mobile phase consisting of methanol-acetonitrile-14.9 mM sodium acetate in water at pH 3.0 (10:23:67, v/v). The detection limit was 10 ng/ml for all the compounds using an ultraviolet detector operated at 230 nm. The method was used to study the pharmacokinetics of midazolam after an intravenous bolus dose (0.75 mg/kg). |
| |
| Keywords: | |
| 本文献已被 ScienceDirect 等数据库收录！ |
|
