Oxytocin receptors in the porcine endometrium during the estrous cycle and early pregnancy |
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| Affiliation: | 1. Department of Animal Reproduction, National Institute of Animal Industry, Tsukuba Norindanchi, Ibaraki 305, Japan;2. Division of Animal Science and Technology, Faculty of Agriculture, Okayama University, Okayama 700, Japan;3. Institute of Physiology, Technical University of Munich, 8050 Freising-Weihenstephan, Germany;1. Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok, 10900, Thailand;2. Swine Reproduction Research Unit, Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, 10330, Thailand;3. Department of Animal Sciences, University of Illinois, Champaign-Urbana, IL, 61801, USA;1. Department of Hepatobiliary Surgery, Zhengzhou University People’s Hospital & Henan Provincial People’s Hospital, Zhengzhou, 450000, PR China;2. Academy of Pharmacy and Chemical Engineering, Zhengzhou University of Industrial Technology, Zhengzhou, 451199, PR China;1. Wheat Genetics Resources Center, Department of Plant Pathology, Throckmorton Plant Sciences Center, Kansas State University, Manhattan, KS 66506, United States;2. Department of Genetics, University of Alabama at Birmingham, Birmingham, AL 35294, United States;3. Department of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, United States;4. College of Osteopathic Medicine, William Carey University, Hattiesburg, MS, United States;5. Department of Pathology, University of Alabama at Birmingham, Birmingham, AL 35294, United States;6. Department of Biostatistics, University of Alabama at Birmingham, Birmingham, AL 35294, United States;7. Departments of Genetics, Pathology, Environmental Health, Center for Free Radical Biology, Center for Aging, UAB Comprehensive Cancer Center, University of Alabama at Birmingham, AL 35294, United States;8. Birmingham Veterans Affairs Medical Center, Birmingham, AL 35294, United States;1. Institute of Cardiovascular Science, University College London, London, United Kingdom;2. Inherited Cardiovascular Disease Unit, St Bartholomew’s Hospital, London, United Kingdom;3. William Harvey Research Institute, Queen Mary University of London, London, United Kingdom;4. Erich and Hanna Klessmann Institute for Cardiovascular Research & Development (EHKI), Heart and Diabetes Center NRW, University Hospital of the Ruhr-University Bochum, Germany;5. Cardiology Clinical Academic Group, St George''s University of London, Cranmer Terrace, London, United Kingdom;6. Department of Pathology, Great Ormond Street Hospital for Children NHS Foundation Trust, London, United Kingdom;7. Experimental Biophysics and Applied Nanoscience, Physics Department, Bielefeld Institute for Nanoscience (BINAS), Bielefeld University, Bielefeld, Germany;1. Department of Production Animal Studies, Faculty of Veterinary Science, University of Pretoria, South Africa;2. Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, South Africa;3. Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, South Africa;4. Pferdezentrum Bad Saarow, Clinic for Horses, Free University, Bad Saarow, Germany;5. Department of Companion Animal Studies, Faculty of Veterinary Science, University of Pretoria, South Africa |
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| Abstract: | Oxytocin (OT) receptors in the porcine endometrium were investigated at four stages of the estrous cycle (Days (D) 0, 5, 10 and 15, n = 3), and at two stages of early pregnancy (D5 and D15 after mating, n = 3) by a radioreceptor assay using 125I-labeled OT antagonist [d(CH2)5,Tyr(Me)2,Thr4,Tyr-NH92]-vasotocin. Binding specificity was demonstrated by displacement with four peptides related to oxytocin ([Arg7]-vasopressin, [Thr4,Gly7]-OT, OVT, OT) and two peptides unrelated to oxytocin (luteinizing hormone-releasing hormone, [Ile3]-pressinoic acid (tocinoic acid)). The dissociation constant (Kd) of endometrial OT receptors on D0 (0.59 ± 0.10 nM) was similar to those on D10 and D15 (D10, 0.75 ± 0.21; D15, 0.60 ± 0.14 nM; mean ± SEM). In the early luteal stage (D5), Kd (2.41 ± 0.24 nM) was higher than on D0, D10 and D15 (P < 0.01). In early pregnancy, Kd values were 3.25 ± 0.29 nM on D5 and 2.44 ± 0.44 nM on D15. Binding site concentration (Bmax) on D0 (910.0 ± 25.1 fmol mg−1 protein) was significantly higher than on D5 and D10 (D5, 322.5 ± 71.7; D10, 147.5 ± 25.8 fmol mg−1 protein; P < 0.01) of the estrous cycle and D5 and D15 (D5, 302.5 ± 82.6; D15, 315.0 ± 20.1 fmol mg−1 protein; P < 0.01) of early pregnancy. In the two stages of early pregnancy, Bmax values were constant and similar to that on D5 of the early luteal stage.Our results reveal the existence of specific OT binding sites in the porcine endometrium during the estrous cycle and early pregnancy. Furthermore, the fluctuation in the binding of OT to the endometrium during the different stages of the estrous cycle suggests that OT plays an important role in regulating the estrous cycle of the pig as seen in other animals. |
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