Simultaneous determination of triamcinolone acetonide and hydrocortisone in human plasma by high-performance liquid chromatography |
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| Affiliation: | 1. ACC, Analytical Clinical Concepts GmbH, Industriestr. 14-16, 64319 Pfungstadt, Germany;2. Harrison Clinical Research GmbH, Albrechtstr. 43, 80636 München, Germany;3. Humboldt-Universität zu Berlin, Math. Nat. I Fak. Institut für Pharmazie Goethestr. 54, 13086 Berlin, Germany;1. Departamento de Farmacología, Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Universidad de Santiago de Compostela, Campus Vida s/n, 15782, Santiago de Compostela, Spain;2. Instituto de Farmacia Industrial, Facultad de Farmacia, Universidad de Santiago de Compostela, Campus Vida s/n, 15782, Santiago de Compostela, Spain;1. Department of Biological Sciences and Bioengineering, Indian Institute of Technology Kanpur, Kanpur 208016, India;2. The Mehta Family Centre for Engineering in Medicine, Indian Institute of Technology Kanpur, Kanpur 208016, India;3. Department of Chemical, Biochemical and Environmental Engineering, University of Maryland Baltimore County, Baltimore, MD 21250, USA;1. AP-HP, Pitié-Salpêtrière Hospital, Department of Pharmacology and CIC-1421, F-75013 Paris, France;2. Sorbonne University, UPMC Univ Paris 06, Faculty of Medicine, Department of Pharmacology and UMR ICAN 1166, F-75013 Paris, France;3. INSERM, CIC-1421 and UMR ICAN 1166, F-75013 Paris, France;1. Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;2. Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran;3. Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran;4. Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran;2. Istituto di Biofisica, Consiglio Nazionale delle Ricerche, Via Ugo La Malfa 153, 90146 Palermo, Italy;3. Advanced Technologies Network Center (ATeN Center), University of Palermo, Viale Delle Scienze – Edificio 18, 90128 Palermo, Italy;4. Dipartimento di Discipline Chirurgiche, Oncologiche e Stomatologiche (DICHIRONS), Università degli Studi di Palermo, Via Liborio Giuffrè 5, 90127 Palermo, Italy |
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| Abstract: | A validated HPLC method for the simultaneous determination of triamcinolone acetonide and hydrocortisone has been established to monitor the plasma levels of the compounds in healthy volunteers following intramuscular (i.m.) administration of triamcinolone acetonide. Plasma (1.0 ml) was extracted with dichloromethane after addition of the internal standard, fluocortolone. The compounds were separated using a LiChrospher RP 18 column and detected by UV absorbance. Specificity, linearity, as well as the repeatability, intermediate precision and accuracy of the method were established. The limit of quantification was 0.6 ng/ml for triamcinolone acetonide (C.V.=8.7%, R.E.=2.6%, n=6) and 2.0 ng/ml for hydrocortisone (C.V.=8.3%, R.E.=2.8%, n=6). Data on the stability of triamcinolone acetonide in human plasma are presented. Recovery of the compounds and the internal standard have been studied. The results of quality control samples (n=126) determined during routine analysis of volunteer samples are described. Plasma levels of triamcinolone acetonide after i.m. administration of 40 mg of triamcinolone acetonide are presented. |
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