Effects of extracellular potassium concentration on meiotic and cytoplasmic maturation in the porcine oocyte |
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| Institution: | 1. School of Human and Life Sciences, Canterbury Christ Church University, Canterbury, UK;2. School of Biosciences, University of Kent, Canterbury, UK;3. JSR Genetics Ltd, Southburn, Driffield, East Yorkshire, UK;1. Center for Advanced Reproductive Services, Division of Reproductive Endocrinology and Infertility, Department of Obstetrics and Gynecology, University of Connecticut Health Center, Farmington, Connecticut;2. Department of Biostatistics, University of Connecticut Health Center, Farmington, Connecticut;1. Laboratory of Reproductive Biology, The Copenhagen University Hospital, Copenhagen, Denmark;2. Faculty of Health and Medical Science, Copenhagen University, Copenhagen, Denmark;3. School of Computer Science, University of St Andrews, St. Andrews, Scotland;4. Department of Obstetrics and Gynecology, University of Medicine and Pharmacy at Ho Chi Minh City, Ho Chi Minh City, Vietnam;5. IVFMD, My Duc Hospital, Ho Chi Minh City, Vietnam;6. HOPE Research Center, My Duc Hospital, Ho Chi Minh City, Vietnam;1. Department of Animal Reproduction, University of José Rosário Vellano, Alfenas, Minas Gerais, Brazil;2. Department of Cell and Developmental Biology, Federal University of Alfenas, Alfenas, Minas Gerais, Brazil;3. University of Western São Paulo, Presidente Prudente, São Paulo, Brazil;1. Department of Laboratory Animal Center, College of Animal Sciences, Jilin University, Changchun, 130062, China;2. Department of Animal Science, Chungbuk National University, Cheongju, Chungbuk, 361-763, Republic of Korea;1. Division of Infertility and Reproductive Endocrinology, Department of Gynecology, Obstetrics and Gynecological Oncology, Poznan University of Medical Sciences, Poznan, Poland;2. Department of Biomaterials and Experimental Dentistry, Poznań University of Medical Sciences, Poznań, Poland;3. Department of Histology and Embryology, Poznan University of Medical Sciences, Poznan, Poland;4. Department of Anatomy, Poznan University of Medical Sciences, Poznan, Poland;5. Department of Obstetrics and Gynecology, University Hospital and Masaryk University, Czech Republic;6. Department of Animal Physiology University of Warmia and Mazury, Olsztyn, Poland;7. Veterinary Center, Nicolaus Copernicus University in Torun, Torun, Poland;8. Department of Pathophysiology, Poznań University of Medical Sciences, Poznan, Poland;9. Department of Histology and Embryology, Wroclaw Medical University, Wroclaw, Poland |
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| Abstract: | Effects of extracellular potassium (K+) concentration in maturation media on the meiotic and cytoplasmic maturation of porcine oocytes were examined. Oocyte-cumulus cell complexes or cumulus cell denuded oocytes were cultured in Whitten's medium containing 0, 3, 6, 12 or 16 mM potassium. Absence of K+ in the media did not inhibit germinal vesicle breakdown (GVBD) in cumulus intact oocytes, but significantly decreased the frequency of meiotic maturation. In cumulus cell denuded oocytes, both GVBD and meiotic maturation were inhibited in K+-free medium. Millimole concentrations of K+ channel blockers, 4-aminopyridine or tetraethyl ammonium chloride inhibited GVBD and almost completely suppressed progression of meiotic maturation. The effect of varying the concentration of K+ on cytoplasmic maturation of pig oocytes was evaluated by the ability to form a male pronucleus after in vitro fertilisation. The percentage of sperm penetration or monospermic penetration was not different among treatments (P > 0.1). However, male pronuclear formation in oocytes in medium with 6 mM K+ was higher than in media with 12 and 16 mM K+. These results suggest that extracellular K+ is required for GVBD and meiotic maturation, and high concentrations (12 or 16 mM) of K+ in maturation media impair cytoplasmic maturation. |
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