Capillary electrophoresis as a clinical tool |
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| Institution: | 1. Institute of Veterinary, Animal and Biomedical Sciences, Massey University, Palmerston North, New Zealand;2. AgResearch Ltd., Ruakura Research Centre, Hamilton, New Zealand;3. Rivalea Australia, Corowa, NSW, Australia;1. Infection Care Group, St George''s University Hospitals NHS Foundation Trust, London, UK;2. Analytical Services, Ministry of Justice, London, UK;3. Infection Prevention and Control Team, St George''s University Hospitals NHS Foundation Trust, London, UK;4. Pharmacy Department, St George''s University Hospitals NHS Foundation Trust, London, UK;5. Department of Microbiology, East Kent Hospitals University NHS Foundation Trust, Ashford, UK;6. Institute of Infection and Immunity, St George''s University of London, London, UK;1. Departament de Bioquímica i Biologia Molecular i Servei de Bioquímica Clínica Veterinària, Facultat de Veterinària, Universitat Autònoma de Barcelona, 08193 Bellaterra (Cerdanyola del Vallés), Spain;2. Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Madrid, Spain;3. Centre de Recerca en Sanitat Animal (CReSA), UAB-IRTA, Campus de la Universitat Autònoma de Barcelona, 08193 Bellaterra (Cerdanyola del Vallés), Spain;4. The Pirbright Institute, Ash Road, Pirbright GU24 0NF, UK;5. Departament de Sanitat i d’Anatomia Animals, Universitat Autònoma de Barcelona, 08193 Bellaterra (Cerdanyola del Vallés), Spain |
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| Abstract: | Clinical laboratories must produce accurate results for patients with a minimum turn-around time. Automated commercial capillary electrophoresis instrumentation has been available to the clinical laboratory for the past five years. Our laboratory has utilised capillary electrophoresis (CE) to automate serum protein electrophoresis. We have used the technique of CE to produce clinical results for nearly two years. CE methods are also available for the quantitation of haemoglobin variants, by both isoelectric focusing and free solution techniques. Micellar electrokinetic separations by CE have been developed for some specialised drug assays and for B-group vitamin analysis, while gel-filled capillaries have the capability to separate DNA fragments, such as PCR products. Isoenzyme analysis has shown possibilities by CE, but quantitative results are needed to be clinically useful. Analysis of amino acids for newborn screening programs and as an arterial clotting indicator are being developed. The next five years should see a proliferation of clinical laboratory methods using automated CE. |
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