Microencapsulation of bovine spermatozoa: effect of capsule membrane thickness on spermatozoal viability and fertility |
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| Affiliation: | 1. Department of Dairy Science, Virginia Polytechnic Institute and State University, Blacksburg, VA 24061-0315, USA;2. Livestock Improvement Corporation Ltd, New Zealand Dairy Board, Private Bag 3016, Hamilton, New Zealand;3. AgResearch, Ruakura Agricultural Centre, Private Bag 3123 Hamilton, New Zealand;1. Department of Veterinary Physiology, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura 281001, Uttar Pradesh, India;2. Department of Veterinary Medicine, College of Veterinary Science & Animal Husbandry, U.P. Pandit Deendayal Upadhayaya Pashu Chikitsa Vigyan Vishwavidyalaya Evam Go Anusandhan Sansthan (DUVASU), Mathura 281001, Uttar Pradesh, India;1. Department of Food Engineering, University of Agriculture, Faisalabad 38000, Pakistan;2. Wageningen University, Food Process Engineering Group, Bornse Weilanden 9, 6708 WG Wageningen, The Netherlands;1. Faculty of Veterinary Medicine, Laboratory of Manipulation of Oocytes and Preantral Follicles (LAMOFOPA), State University of Ceará, Fortaleza, CE, Brazil;2. Institute of Health Sciences, University of International Integration Lusophone African-Brazilian, Acarape, CE, Brazil;3. Federal University of Uberlândia, MG, Brazil;4. Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Beaverton, OR, USA;1. LUNAM University, Oniris, Nantes-Atlantic National College of Veterinary Medicine, Food Science and Engineering, Sanitary Security of Reproduction Biotechnology Unit, Nantes, France;2. INRA, Animal Infectious Diseases and Public Health Tours, France;3. ANSES, Animal Health Laboratory, Bacterial Zoonosis Unit, Maisons-Alfort, France |
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| Abstract: | This study was undertaken to investigate the relationship between the cationic polymer (poly-L-lysine) concentration and microcapsule membrane thickness, maintenance of spermatozoal motility in vitro, and pregnancy rate in 335 oestrous synchronized Friesian heifers. Semen was extended in CAPROGEN™ containing 5% egg yolk and a final encapsulated spermatozoal concentration of 20 × 106 spermatozoa ml−1. Four concentrations of poly-L-lysine were studied (0.025, 0.05, 0.075, and 0. 1%, w/v). Microcapsule membrane thickness resulting from these concentrations was 3.22 ± 0.54 μm (mean ± SD), 5.30 ± 0.31 μm, 7.12 ± 0.41 μm, and 7.44 ± 0.85 μm, respectively (P < 0.05). Spermatozoal viability, as assessed by motility estimates at 24 h intervals during 120 h of incubation at 37°C, was not influenced by polymer concentration or different than unencapsulated controls. For fertility evaluation approximately 65 Friesian heifers were inseminated with spermatozoa either unencapsulated or encapsulated with one of the four polymer concentrations. Oestrous synchronization was accomplished with the combination of a progesterone-impregnated CIDR-B® device containing a 10 mg oestradiol benzoate capsule inserted for 10 days with administration of 12.5 mg of prostaglandin F2α on Day 6 of CIDR-B® insertion. Heifers were inseminated in the uterine corpus at 24 h after CIDR-B® removal which constituted the pro-oestrous stage of the cycle for 95.5% of the heifers. Inseminate dose rate was 5 × 106 spermatozoa in 0.25 ml. Pregnancy rates were similar for heifers inseminated with encapsulated and unencapsulated spermatozoa (49.4 vs. 48.6%).From these studies we conclude that poly-L-lysine concentration does influence the microcapsule membrane thickness without affecting maintenance of spermatozoal motility in vitro or fertility of oestrous synchronised Friesian heifers. |
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