Determination of insulin in single pancreatic cells by capillary electrophoresis and laser-induced native fluorescence |
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| Institution: | 1. Department of Pharmacy and Biotechnology, University of Bologna, Via Belmeloro 6, Bologna 40126, Italy;2. Department of Chemistry “Giacomo Ciamician”, University of Bologna, Via F. Selmi 2, Bologna 40126, Italy;3. Department of Chemistry “Ugo Schiff”, University of Florence, Via U. Schiff 6, Florence, Sesto Fiorentino 50019, Italy;4. Department of Biological, Geological and Environmental Sciences, University of Bologna, Via S. Alberto 163, Ravenna 48123, Italy |
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| Abstract: | Development of a method for the determination of insulin based on capillary electrophoresis with laser-induced native fluorescence detection is described. Under optimal conditions, insulin as low as 73 amol can be detected with a good signal-to-noise ratio (S/N=10 peak-to-peak). Application of this method for the determination of insulin content in single cells from the insulin-secreting cell lines RINm5F and βTC3 is demonstrated. Non-bonded poly(ethylene oxide)-coated and bare capillaries are evaluated for this purpose, with the latter found to be more suitable for single-cell analysis. |
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