Manual and automated (robotic) high-performance liquid chromatography methods for the determination of mycophenolic acid and its glucuronide conjugate in human plasma |
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| Institution: | 1. Department of Industrial Engineering, University Of Padova, Padova 35131, Italy;2. Department of Chemistry, Bharathiar University, Coimbatore 641046, Tamil Nadu, India;3. Department of Chemistry, Texas A&M University, College Station, TX 77842, USA;1. JMI Laboratories, North Liberty, Iowa, USA;2. University of Iowa College of Medicine, Iowa City, Iowa, USA;1. Liaoning Laboratory of Cancer Genetics and Epigenetics and Department of Cell Biology, College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, China;2. Department of Gynaecological Surgery, Sheng-Jing Hospital, China Medical University, Shenyang 110004, China;3. Department of Clinical Oncology, Second Affiliated Hospital of Dalian Medical University, Dalian 110042, China |
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| Abstract: | A manual and an automated (Zymark PyTechnology robot) HPLC method for simultaneous determination of plasma mycophenolic acid (MPA) and its glucuronide conjugate (MPAG) are described here. Both methods are reproducible and accurate, and both are equivalent in all respects, including quantification limits (MPA, 0.100 μg/ml; MPAG, 4.00 μg/ml), range (using 0.05–0.5 ml of plasma: MPA, 0.0500–20.0 μg/aliquot; MPAG, 2.00–200 μg/aliquot), precision, and accuracy. MPA and MPAG were stable under the conditions used with both methods. Results from aliquots of paired control samples, analyzed by the manual method over three years at six analytical laboratories, showed excellent agreement in precision and accuracy. |
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