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Sensitive determination of bromazepam in human tissues using capillary gas chromatography-mass spectrometry
Affiliation:1. National Centre of Excellence in Analytical Chemistry, University of Sindh, Jamshoro 76080, Pakistan;2. Dr M.A. Kazi Institute of Chemistry University of Sindh, Jamshoro 76080, Pakistan;1. College of Chemistry and Chemical Engineering, State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China;2. College of Chemical Engineering and Technology, Tianshui Normal University, TianShui, GanSu 741001, China;1. Faculty of Chemistry, Razi University, Kermanshah 671496734, Iran;2. Laboratorio de Desarrollo Analítico y Quimiometría (LADAQ), Cátedra de Química Analítica I, Universidad Nacional del Litoral, Ciudad Universitaria, CC 242 (S3000ZAA) Santa Fe, Argentina;3. Solution Equilibria and Chemometrics Group, Department of Analytical Chemistry, University of Barcelona, Diagonal 645, E-08028 Barcelona, Spain;4. Quality & Technology, Department of Food Science, Faculty of Science, University of Copenhagen, Copenhagen, Denmark
Abstract:A reliable and sensitive gas chromatographic-mass spectrometric method was devised to determine the levels of bromazepam in human tissues. Bromazepam was extracted from body tissues using a three-step solvent extraction procedure. N-Desmethyldiazepam served as the internal standard. Selected ion monitoring withm/z 317 for bromazepam andm/z 270 for internal standard was used for quantitation. Calibration curves in all body tissues were linear over the concentration range from 50–500 ng/g. The lower detection limit in body tissues was 2–5 ng/g and the absolute recovery in body tissues was 27.8–68.0%. This method was used to determine the levels of bromazepam in tissues of an autopsied individual who had been prescribed psychotropic drugs and who was found dead in a car.
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