| 响应面分析法优化耐高温蛋白酶发酵培养基 |
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| 引用本文： | 周虓,郑毅,叶海梅,石磊. 响应面分析法优化耐高温蛋白酶发酵培养基[J]. 生物数学学报, 2007, 22(1): 113-118 |
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| 作者姓名： | 周虓 郑毅 叶海梅 石磊 |
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| 作者单位： | 1. 福建师范大学,工业微生物教育部工程研究中心,福建,福州,350007;呼和浩特市疾病预防控制中心,内蒙古,呼和浩特,010070
2. 福建师范大学,工业微生物教育部工程研究中心,福建,福州,350007 |
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| 摘 要： | 采用响应面分析方法对产耐高温蛋白酶的苏云金芽孢杆菌(Bacillus thuringien- sis)FZ62发酵培养基进行优化．首先,进行发酵培养基碳源、氮源及初始pH值的单因素筛选,优化结果表明最适氮源为酵母粉、碳源是葡萄糖,初始pH值范围6-8．在此基础经响应面法优化,发酵培养基最佳组合为:酵母粉2．04%,葡萄糖为0．10%,初始pH7．07．经以上优化后发酵水平比初始设计提高了3．22倍．
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| 关 键 词： | 响应面分析方法 耐高温蛋白酶 苏云金芽孢杆菌 |
| 文章编号： | 1001-9626（2007）01-0113-06 |
| 收稿时间： | 2006-01-07 |
| 修稿时间： | 2006-01-072006-09-24 |
Fermentation Medium Optimization of Thermophilic Proteinase by Response Surface Methodology |
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| ZHOU Xiao,ZHENG Yi,YE Hai-mei,SHI Lei. Fermentation Medium Optimization of Thermophilic Proteinase by Response Surface Methodology[J]. Journal of Biomathematics, 2007, 22(1): 113-118 |
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| Authors: | ZHOU Xiao ZHENG Yi YE Hai-mei SHI Lei |
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| Affiliation: | 1 Engineering Research Center of Industrial Microbiology. Ministry of Education, Fujian Normal Uinversity, Fuzhou Fujian 3500070 China;2 Huhhot Center for Disease Control and Prevention, Inner Mongolia 010070 China |
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| Abstract: | Response Surface Methodology(RSM)was used to optimize fermentation medium of Bacillus thuringiensis FZ62 producing Thermophilic proteinase.First,Carbon source,Nitro- gen source and initial pH value of fermentation.medium were optimized independently,the result showed that the best Carbon and Nitrogen source were glucose and yeast powder respectively , the range of initial pH value was 6-8.The optimal fermentation medium component obtained by RSM was yeast powder2.04%,glucose 0.1% and initial pH 7.07.After the optimization,the yield of thermophilic proteinase by FZ62 increased 3.22 times. |
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| Keywords: | Response surface methodology Thermophilic proteinase Bacillus thuringiensis |
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