Site-directed RNA editing (SDRE): Off-target effects and their countermeasures |
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| Authors: | Shaoshuai Mao Yajing Liu Shisheng Huang Xingxu Huang Tian Chi |
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| Affiliation: | 1. School of Life Sciences and Technology, ShanghaiTech University, Shanghai, 201210, China;2. CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, 200031, China;3. University of Chinese Academy of Sciences, Beijing, 100049, China;4. Department of Immunobiology, Yale University School of Medicine, New Haven, CT 06520, USA |
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| Abstract: | Site-directed RNA editing (SDRE) is invaluable to basic research and clinical applications and has emerged as a new frontier in genome editing. The past few years have witnessed a surge of interest in SDRE, with SDRE tools emerging at a breathtaking pace. However, off-target effects of SDRE remain a tough problem, which constitutes a major hurdle to their clinical applications. Here we discuss the diverse strategies for combating off-target editing, drawing lessons from the published studies as well as our ongoing research. Overall, SDRE is still at its infancy, with significant challenges and exciting opportunities ahead. |
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| Keywords: | Corresponding author. Gene editing RNA editing Cas13 Off-target Specificity |
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