A Comparison of Protein and mRNA Expression during Development of the Soil Dwelling Prokaryote (S. coelicolor) |
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| Authors: | Jiří Pospíšil Dmytro Strunin Alice Ziková Martin Hubálek Jiří Vohradský |
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| Affiliation: | 1. Laboratory of Bioinformatics, Institute of Microbiology, Czech Academy of Sciences, v.v.i., Videnska 1083, Prague, 14220 Czech Republic;2. Institute of Organic Chemistry and Biochemistry, Czech Academy of Sciences, v.v.i., Flemingovo n. 2, Prague, 16610 Czech Republic;3. E‐mail: |
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| Abstract: | In this paper, correlation analysis of protein and mRNA levels in the soil dwelling bacteria Streptomyces coelicolor (S. coelicolor M145) is presented during development of the population as it grew in liquid medium using three biological and two technical replicates, measured during exponential growth, and its entry into the stationary phase. The proteome synthesis time series are compared with the gene expression time series measured previously under identical experimental conditions. Results reveal that about one third of protein/mRNA synthesis profiles are well correlated while another third are correlated negatively. Functional analysis of the highly correlated groups is presented. Based on numerical simulation, the negative correlation between protein and mRNA is shown to be caused by the difference between the rate of translation and protein degradation. |
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| Keywords: | computational modeling mass spectrometry mRNA/protein expression proteins functional analysis Streptomyces |
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