Changes in the Bacterial Microbiota in Gut,Blood, and Lungs following Acute LPS Instillation into Mice Lungs |
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Authors: | Marc A. Sze Masashi Tsuruta Shun-Wei Julia Yang Yeni Oh S. F. Paul Man James C. Hogg Don D. Sin |
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Affiliation: | 1. Heart + Lung Innovation Centre at St. Paul''s Hospital, University of British Columbia, Vancouver, BC, Canada.; 2. Departments of Medicine, Respiratory Division, University of British Columbia, Vancouver, BC, Canada.; 3. Pathology and Laboratory Medicine, University of British Columbia, Vancouver, BC, Canada.; University Hospital Freiburg, Germany, |
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Abstract: | IntroductionPrevious reports have shown that the gastrointestinal (GI) bacterial microbiota can have profound effects on the lungs, which has been described as the “gut-lung axis”. However, whether a “lung-gut” axis exists wherein acute lung inflammation perturbs the gut and blood microbiota is unknown.MethodsAdult C57/Bl6 mice were exposed to one dose of LPS or PBS instillation (n = 3 for each group) directly into lungs. Bacterial microbiota of the bronchoalveolar lavage fluid, blood, and cecum were determined using 454 pyrotag sequencing and quantitative polymerase chain reaction (qPCR) at 4 through 168 hours post-instillation. We then investigated the effects of oral neomycin and streptomycin (n = 8) on the microbiota at 4 and 24 hours post LPS instillation versus control treatment (n = 5 at baseline and 4 hours, n = 7 at 24 hours).ResultsAt 24 hours post LPS instillation, the total bacterial count was significantly increased in the cecum (P<0.05); whereas the total bacterial count in blood was increased at 4, 48, and 72 hours (P<0.05). Antibiotic treatment reduced the total bacteria in blood but not in the cecum. The increase in total bacteria in the blood correlated with Phyllobacteriaceae OTU 40 and was significantly reduced in the blood for both antibiotic groups (P<0.05).ConclusionLPS instillation in lungs leads to acute changes in the bacterial microbiota in the blood and cecum, which can be modulated with antibiotics. |
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