Stigmasterol isolated from marine microalgae Navicula incerta induces apoptosis in human hepatoma HepG2 cells |
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| Authors: | Young-Sang Kim Xi-Feng Li Kyong-Hwa Kang BoMi Ryu Se Kwon Kim |
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| Affiliation: | 1.Department of Chemistry, Pukyong National University, Busan 608-737, Korea;2.Chemical Biology Center of YanBian University, Yanji, Jilin 133002, China;3.Marine Bioprocess Research Center, Pukyong National University, Busan 608-737, Korea;4.School of Pharmacy, The University of Queensland, Brisbane, Qld 4072, Australia;5.Specialized Graduate School Science and Technology Convergence, Department of Marine Bio Convergence Science., Pukyong National University, Busan 608-737, Korea |
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| Abstract: | Plant sterols have shown potent anti-proliferative effects and apoptosis induction against breast and prostate cancers. However, the effect of sterols against hepatic cancer has not been investigated. In the present study, we assessed whether the stigmasterol isolated from Navicula incerta possesses apoptosis inductive effect in hepatocarcimona (HepG2) cells. According to the results, Stigmasterol has up-regulated the expression of pro-apoptotic gene expressions (Bax, p53) while down-regulating the anti-apoptotic genes (Bcl-2). Probably via mitochondrial apoptosis signaling pathway. With the induction of apoptosis caspase-8, 9 were activated. The DNA damage and increase in apoptotic cell numbers were observed through Hoechst staining, annexin V staining and cell cycle analysis. According to these results, we can suggest that the stigmasterol shows potent apoptosis inductive effects and has the potential to be tested as an anti-cancer therapeutic against liver cancer. [BMB Reports 2014; 47(8): 433-438] |
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| Keywords: | Apoptosis Bcl-2 family Caspase8 9 Marine microalgae Navicula incerta Stigmasterol |
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