In Vivo image Analysis Using iRFP Transgenic
Mice |
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| Authors: | Mai Thi Nhu Tran Junko Tanaka Michito Hamada Yuka Sugiyama Shota Sakaguchi Megumi Nakamura Satoru Takahashi Yoshihiro Miwa |
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| Institution: | 1) Department of Anatomy and Embryology, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki 305-8575, Japan;2) Department of Molecular Pharmacology, University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki 305-8575, Japan;3) International Institute for Integrative Sleep Medicine (WPI-IIIS), University of Tsukuba, 1-1-1, Tennodai, Tsukuba, Ibaraki 305-8575, Japan |
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| Abstract: | Fluorescent proteins with light wavelengths within the optical window are one of the
improvements in in vivo imaging techniques. Near-infrared (NIR)
fluorescent protein (iRFP) is a stable, nontoxic protein that emits fluorescence within
the NIR optical window without the addition of exogenous substrate. However, studies
utilizing an in vivo iRFP model have not yet been published. Here, we
report the generation of transgenic iRFP mice with ubiquitous NIR fluorescence expression.
iRFP expression was observed in approximately 50% of the offspring from a matings between
iRFP transgenic and WT mice. The serum and blood cell indices and body weights of iRFP
mice were similar to those of WT mice. Red fluorescence with an excitation wavelength of
690 nm and an emission wavelength of 713 nm was detected in both newborn and adult iRFP
mice. We also detected fluorescence emission in whole organs of the iRFP mice, including
the brain, heart, liver, kidney, spleen, lung, pancreas, bone, testis, thymus, and adipose
tissue. Therefore, iRFP transgenic mice may therefore be a useful tool for various types
of in vivo imaging. |
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| Keywords: | fluorescent protein in vivo imaging iRFP optical window |
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