Media and genotype effects on the development and conversion of somatic alfalfa (Medicago sativa L.) embryos |
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| Authors: | S Hindson A R McElroy C Portelance |
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| Institution: | (1) PARC, Agriculture and Agri-Food Canada, Summerland, BC, Canada;(2) FCORC, Agriculture and Agri-Food Canada, Building #12, KIA 0C6 Ottawa, Ontario, Canada;(3) Canadian Food Inspection Agency, Plant Products Division, 59 Camelot Drive, K1A 0Y9 Nepean, Ontario, Canada |
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| Abstract: | Summary Various preconditioning treatments of alfalfa (Medicago sativa L.) somatic embryos to improve embryo quality and conversion were studied. Four different regenerating genotypes were compared.
Embryogenic cultures were established in liquid culture. Globular embryos were collected and plated on an embryo development
medium until they reached cotyledonary stage. They were then exposed to three treatments: a standard embryo development medium
(control), media supplementation with 1 μM abscisic acid (ABA), 50 mM glutamine and 5% sucrose (T), additional supplementation with 50 μM ABA (TT), and additional supplementation followed by desiccation (TTD). Treatments affected embryo conversion, but not uniformly
for all genotypes. Embryo conversion was increased (P<0.05) by pretreatment (T), while only one exhibited any response to additional ABA (T vs. TT). Desiccation decreased (P<0.05) conversion of pretreated embryos (TT vs. TTD) of all genotypes. The effect of treatments on plantlet weight was less
pronounced and inconsistent across genotypes. |
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| Keywords: | alfalfa Medicago sativa somatic embryogenesis |
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