Structural analysis of the rDNA intergenic spacer of Tuber borchii |
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Authors: | Ciarmela Pasquapina Potenza Lucia Zeppa Sabrina Cucchiarini Luigi Stocchi Vilberto |
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Affiliation: | Istituto di Chimica Biologica Giorgio Fornaini, Universit degli Studi di Urbino, Via A. Saffi 2, 61029 Urbino (PU), Italy. |
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Abstract: | The sequence and characterisation of the entire nuclear rDNA intergenic spacer (IGS) for the genus Tuber are presented. Sequence analyses showed that the organisation of the Tuber borchii rDNA IGS is typical of rDNA spacers, consisting of a central repetitive region and flanking unique sequences on either side. Direct repeats, symmetry elements, tandem repeats and possible areas of recombination were found. The putative ends of the 25S and 17S rDNA were identified. The presence of 5S rDNA in the IGS region was excluded. |
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