| 毛细管水解及反相高效液相色谱分析蛋白质的氨基酸组成 |
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| 引用本文： | 陈平,梁宋平.毛细管水解及反相高效液相色谱分析蛋白质的氨基酸组成[J].中国生物化学与分子生物学报,1996,12(1):112-115. |
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| 作者姓名： | 陈平 梁宋平 |
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| 作者单位： | 湖南师范大学生物研究所 |
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| 摘 要： | 毛细管水解及反相高效液相色谱分析蛋白质的氨基酸组成陈平，梁宋平（湖南师范大学生物研究所，长沙４１０００６）氨基酸组成的分析是阐明蛋白质和多肽化学特性的基础，在蛋白质与多肽的氨基酸组成分析中，常采用对水解管反复充氮并抽真空的方法使蛋白质和多肽在隔绝氧气...
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| 收稿时间： | 1996-02-20 |
A Method for Complete Amino Acid Analysis of Proteins and Peptides by Reverse-Phase High-Performance Liquid Chromatography through Hydrolysis in a Microcapillary Tube |
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| Chen Ping,Liang Song-Ping.A Method for Complete Amino Acid Analysis of Proteins and Peptides by Reverse-Phase High-Performance Liquid Chromatography through Hydrolysis in a Microcapillary Tube[J].Chinese Journal of Biochemistry and Molecular Biology,1996,12(1):112-115. |
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| Authors: | Chen Ping Liang Song-Ping |
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| Institution: | (Institute of Biology, Hunan Normal University, Changsha 410006 |
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| Abstract: | A method of amino acid analysis of proteins and peptides through hydrolysis in a microcapillary tube with methanesulfonic acid containing dithiediglycolic.acid is reported. Hydrolysis in a microcapillary tube eliminates the necessity of time-consuming sealing under vacuum and flushing with nitrogen- High yield of derivatization of cysteine or half-cystine by dithiodiglycolic acid has been achieved and the recovery of tryptophan is greater than 90% by this method. |
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| Keywords: | Microcapillary tube hydrolysis PTC-amino acid Reverse-phase high-performance liquid chromatography |
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