EVALUATION OF BEADRETRIEVERTM, AN AUTOMATED SYSTEM FOR CONCENTRATION OF ESCHERICHIA COLI O157 FROM ENRICHMENT CULTURES BY IMMUNOMAGNETIC SEPARATION |
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| Authors: | PA CHAPMAN KS CUDJOE |
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| Institution: | Public Health Laboratory Herries Road Sheffield S5 7BQ, UK;Dynal Biotech ASA c/o Department of Pharmacology Microbiology and Food Hygiene Norwegian College of Veterinary Medicine P.O. Box 8146 Dep. 0033 Oslo, Norway |
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| Abstract: | The aim of this study was to compare automated immunomagnetic separation (AIMS) with manual IMS (MIMS) for detection of E. coli O157 in natural and artificially inoculated samples. E. coli O157 was isolated from 27 (14.7%) of 184 natural bovine fecal samples by MIMS and from 26 (14.1%) by AIMS. Of 94 natural carcass samples, E. coli O157 was isolated from 6 by both MIMS and AIMS and from a further 6 by AIMS only. Three of the six positive only by AIMS were from tubes processed adjacently to one containing an inoculated sample and strains isolated from these adjacent tubes were indistinguishable by various typing techniques. Of the 36 carcass samples inoculated with E. coli O157, the organism was isolated from 25 by both MIMS and AIMS and from a further 9 by AIMS only. To process 100 samples by MIMS took 5h whereas by AIMS this took only 3h 20 min which represented a considerable saving of staff time. |
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