Effects of brassinosteroid infiltration prior to cold treatment on ion leakage and pigment contents in rape leaves |
| |
Authors: | A Janeczko G Gullner A Skoczowski F Dubert B Barna |
| |
Institution: | (1) The Franciszek Górski Institute of Plant Physiology, Polish Academy of Sciences, 21 Niezapominajek St., PL-30239 Krakow, Poland;(2) Plant Protection Institute, Hungarian Academy of Sciences, 15 Herman Otto St., H-1022 Budapest, Hungary |
| |
Abstract: | The effect of 24-epibrassinolide (BR27) on cold resistance of rape seedlings was studied by ion leakage and photosynthetic pigment degradation measurements. Aqueous
solutions of BR27 were injected into cotyledons or primary leaves of rape plants and these plants were incubated at 2 °C or 20 °C. Cold treatment
(2 °C) without BR27 injection elevated the membrane permeability in both primary leaves and cotyledons significantly. Surprisingly, injection
of leaves with water or 0.467 % aqueous ethanol solution led to a massive increase in membrane permeability after cold stress
at 2 °C. The synergistic effect of leaf infiltration and cold on permeability was abolished by 0.05 and 1.00 μM of BR27 in primary leaves and by 1.00 μM of BR27 in cotyledons. On the other hand, BR27 solutions strongly elevated the membrane permeability at 20 °C, while water and ethanol solutions brought about only negligible
increases. Water or ethanol infiltrations strongly reduced the leaf contents of chlorophyll (Chl) a, Chl b and carotenoids at 2 °C but less markedly at 20 °C. However, in seedlings exposed to 2 °C pigments content was significantly
higher in BR27-treated leaves as compared to water/ethanol control. There were no differences between pigment contents of leaves injected
with BR27 solutions or only water/ethanol at 20 °C. The above data strongly support the stress protecting effect of BR27. |
| |
Keywords: | carotenoids chlorophyll cold stress 24-epibrassinolide ion leakage |
本文献已被 SpringerLink 等数据库收录! |
|