Preparation of gram quantities of a purified R-factor-mediated penicillinase from Escherichia coli strain W3310 |
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| Authors: | J. Melling and G. K. Scott |
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| Affiliation: | Microbiological Research Establishment, Porton, Salisbury, Wilts., U.K.;Department of Molecular Biology, University of Edinburgh, Kings Buildings, Mayfield Road, Edinburgh EH9 3JR, U.K. |
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| Abstract: | Purified penicillinase, in gram quantities, has been prepared from Escherichia coli strain W3310 by using methods developed to handle large amounts of material. The final product had a specific enzyme activity of 3.08 units/mug of protein, which was over twice as high as that reported previously (Datta & Richmond, 1966). The purified enzyme was similar to that from E. coli strain TEM, but different in molecular weight and some other respects. The differences observed may be a result of the greater purity obtained. |
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