Secretion and affinity purification of glutathione S-transferase fusion proteins from yeast |
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| Authors: | L A Castelli A J Petris S M Carroll I G Macreadie |
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| Institution: | (1) Biomolecular Research Institute, 343 Royal Parade, 3052 Parkville, Victoria, Australia;(2) AMRAD Operations, 34 Wadhurst Drive, 3155 Boronia, Victoria, Australia |
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| Abstract: | Summary Sequences encoding GST-fusion proteins were cloned into the Saccharomyces cerevisiae secretion vector, pYEX-S1, to direct secretion into the culture medium. GST and metallothionein fused to GST were secreted successfully and the fusion proteins purified. With several other GST-fusion proteins however, the proteins were retained inside the cell, indicating limitations to the types of proteins that can be secreted from yeast. |
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