Auxin-stimulated RNA Synthesis in Oat Coleoptile Cells |
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| Authors: | Yoshio Masuda Eiichi Tanimoto Shunji Wada |
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| Affiliation: | Department of Biology, Faculty of Science Osaka City University, Sumiyoshi-ku, Osaka, Japan and Department of Biology, Faculty of Science Kobe University, Nada-ku, Kobe, Japan |
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| Abstract: | Using oat coleoptile segments the following results were obtained. Ten mg/l auxin (indole-3-acetic acid) increased the incorporation of uracil-2-14C and orthophosphate-32P into RNA fraction during a relatively short incubation period. Stimulation of 32P incorporation due to auxin was found only in the region heavier than ribosomal RNA, probably in the messenger RNA region. The stimulation of uracil-2-14C incorporation into RNA caused by auxin was not influenced by the presence of 0.3 M mannitol which prevents osmotically the water absorption of cells. It is concluded that auxin primarily stimulates the biosynthesis of RNA, possibly messenger, in oat coleoptile cells. |
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