Lactation-associated redistribution of the glial fibrillary acidic protein within the supraoptic nucleus |
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Authors: | Salm Adrienne K Smithson Kenneth G Hatton Glenn I |
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Institution: | (1) The Neuroscience Program and Department of Psychology, Michigan State University, East Lansing, Michigan, USA;(2) The Neuroscience Program and Department Psychology, Michigan State University, 48824 1117 East Lansing, Michigan, USA |
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Abstract: | Summary Three clones of myeloproliferative virus (MPV)-transformed rat fibroblasts (NRK) with different growth properties and morphology were transplanted to athymic nude mice. Presence of carbohydrate-binding proteins was inferred by fluorescence microscopy using fluorescent, glycosylated markers. Salt and detergent extracts of tumors from this model system were fractionated under identical conditions on different sets of Sepharose columns, to which lactose, asialofetuin, melibiose, mannan and fucose had been covalently linked. Successive elution by chelating reagent and specific sugar resulted in isolation of the different Ca2+-dependent and Ca2+-independent endogenous carbohydrate-binding proteins that were assayable as agglutinins. In comparison, the different tumors displayed a pattern with qualitative and quantitative alterations. Since protein-carbohydrate interaction mediated by carbohydrate-binding proteins (lectins) is of importance for cognitive processes, it is remarkable that the pattern of membrane glycoproteins, isolated by affinity chromatography on resins with immobilized plant lectins, had also been found to reveal certain individual properties for receptors specific for peanut agglutinin (PNA) and Ulex europaeus agglutinin (UEA). These demonstrated differences within the system of protein-carbohydrate interaction suggest that endogenous lectins and their ligands have potential significance as markers defining a certain phenotype within this tumor model system.Dedicated to Prof. Dr. W. Lamprecht on the occasion of his 60th birthday |
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Keywords: | Astrocytes Image analysis Glial fibrillary acidic protein Lactation Supraoptic nucleus Rat |
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