Selective precipitation of phosphofructokinase,glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase from rat erythrocyte hemolysates by polyethylene glycol |
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Authors: | M C Tejedor C Delgado A Rubio J Luque |
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Institution: | (1) Departamento de Bioquimica, F. Ciencias y Farmacia, U. Alcalá de Henares, Madrid, Spain |
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Abstract: | Precipitation profiles of phosphofructokinase, glucose-6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase have been established in the range of 0–16% PEG at different pH (5–7) values. Precipitation generally occurred between narrow limits of polyethylene glycol. The polymer concentration needed to reach any level of enzyme precipitation is dependent on pH. Particular conditions (% PEG and pH) for the selective enzyme enrichment have been determined. |
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Keywords: | PEG PFK G6PD 6PGD rat erythrocytes |
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