Cometabolic biodegradation of methyl t-butyl ether by Pseudomonas aeruginosa grown on pentane |
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Authors: | P M Garnier R Auria C Augur S Revah |
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Institution: | ORSTOM (Institut Fran?ais de Recherche Scientifique pour le Développement en Coopération), Cicerón 609, Los Morales 11530, Mexico D.F., Mexico, MX Department of Chemical Engineering, Universidad Autónoma Metropolitana-Iztapalapa, Apdo. Postal 55-534, 09340 Mexico D.F., Mexico e-mail: srevah@xanum.uam.mx, MX
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Abstract: | A bacterial strain identified as Pseudomonas aeruginosa was isolated from a soil consortium able to mineralize pentane. P. aeruginosa could metabolize methyl t-butyl ether (MTBE) in the presence of pentane as the sole carbon and energy source. The carbon balance for this strain, grown
on pentane, was established in order to determine the fate of pentane and the growth yield (0.9 g biomass/g pentane). An inhibition
model for P. aeruginosa grown on pentane was proposed. Pentane had an inhibitory effect on growth of P. aeruginosa, even at a concentration as low as 85 μg/l. This resulted in the calculation of the following kinetic parameters (μmax = 0.19 h−1, K
s = 2.9 μg/l, K
i = 3.5 mg/l). Finally a simple model of MTBE degradation was derived in order to predict the quantity of MTBE able to be degraded
in batch culture in the presence of pentane. This model depends only on two parameters: the concentrations of pentane and
MTBE.
Received: 16 July 1998 / Received revision: 11 November 1998 / Accepted 31 November 1998 |
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