Increase of callus and embryoid production from hypocotyl protoplasts of sunflower (Helianthus annuus L.) by culture in microdrops |
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| Authors: | J ?amaj A Okolot M Bobak Yu Gleba |
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| Institution: | 1. Department of Plant Physiology, Comenius University, Mlynská dolina B-2, 842 15, Bratislava, Slovak Republic
2. Institute of Cell Biology and Engineering, Lebedeva Street 1, 252 650, Kiev GSP 22, Ukraine
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| Abstract: | Hypocotyls of seedling plants of the sunflower inbred line HNK-81 were used as a source of protoplasts. Optimal conditions
of isolation and culture of protoplasts were established. Using the method of individual culture in microdrops, a 77% final
plating efficiency was achieved. Cytokinins, especially zeatin, showed a significant influence on the formation of globular
embryo-like structures, but these failed to develop into mature embryos. Calli obtained in liquid media containing auxins
and cytokinins grew on agar solidified media without growth regulators.
Communicated by M. ONDŘEJ |
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