单细胞测序分析人类胚胎干细胞神经分化的分子机制

目的 探讨人类胚胎干细胞(ESCs)分化为神经细胞的关键性靶基因及分子机制,为临床靶向治疗神经康复患者提供分子理论依据.方法 基于GEO数据平台芯片,采用单细胞测序方法(scRNA-seq),利用R语言从多分子维度(单细胞差异基因、蛋白互作网络和基因通路等)分析人类ESCs分化过程中的关键Marker基因并利用质控和数...

Molecular mechanism of neural differentiation in human embryonic stem cells by single-cell sequencing analysis

Objective To explore the key target genes and molecular mechanisms of human embryonic stem cell differentiation into neural cells,and provide molecular theoretical basis for clinical targeted treatment of neurological rehabilitation patients.Methods Based on the chip data from GEO platform,the key Marker genes in the differentiation process of human embryonic stem cells were analyzed from the multi-molecular dimension(single cell differential genes,protein interaction networks,gene pathways,etc.)by using R language as well as using single cell sequencing method.Quality control and data filtering,PCA,TSNE analysis,cell trajectory analysis,GO enrichment analysis,KEGG enrichment analysis,KEGG pathway analysis were used to show the mechanism of Marker gene regulating human embryonic stem cell differentiation.Results GO functional enrichment analysis showed that Marker genes played a significant role in germ layer differentiation,extracellular matrix and signal transduction.Marker gene interaction network and KEGG pathway showed that characteristic Fibronectin 1(FN1),Nanog homeobox(NANOG)and Growth factor receptor-bound protein 2(GRB2)genes were the key genes.KEGG pathway analysis showed that FN1 played a significant role in regulating extracellular matrix pathway,and NANOG and GRB2 played significant roles in regulating stem cell pluripotency signaling pathway.Conclusion FN1 may mediate the matrix environment of human embryonic stem cells by regulating extracellular matrix pathways and up-regulation of NANOG and GRB2 in stem cell pluripotency signaling pathway could mediate directional differentiation of human embryonic stem cells into neural tissue.