Fluid Phase Endocytic Uptake of Artificial Nano-Spheres and Fluorescent Quantum Dots by Sycamore Cultured Cells: Evidence for the Distribution of Solutes to Different Intracellular Compartments |
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| Authors: | Ed Etxeberria Pedro Gonzalez Edurne Baroja-Fernandez Javier Pozueta Romero |
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| Institution: | 1.University of Florida; Institute of Food and Agricultural Sciences; Citrus Research and Education Center; Department of Horticultural Sciences; Lake Alfred, Florida USA;2.Agrobioteknologia Instituta; Consejo Superior de Investigaciones Cientificas and Nafarroako Unibertsitate Publikoa; Mutiloako etorbidea zembaki gabe; Mutiloabeti, Nafarroa, Spain |
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| Abstract: | Fluid phase endocytic uptake of external solutes in plant cells was further substantiated using artificial polystyrene nano-spheres (40 nm) and CdSe/ZnS quantum dots (20 nm). Both types of artificial nano-particles were taken up by sycamore-cultured cells. However, whereas polystyrene nano-spheres were delivered to the central vacuole, CdSe/ZnS nano-dots were sequestered into cytoplasmic vesicular structures. Using dextran-Texas Red (m.w. 3,000; d-TR) as additional marker, confocal micrographs confirmed the distinct topographic distribution of CdSe/ZnS quantum dots within the cell. Initially, d-TR and CdSe/ZnS quantum dots colocalized within cytoplasmic vesicles. After 18 h incubation, d-TR was distinctly localized in the vacuole whereas CdSe/ZnS quantum dots remained sequestered in cytoplasmic membranous compartments. The data provide a first evidence for the rapid distribution of solutes taken up by endocytosis to distinct intracellular compartments.Key Words: apoplast, assimilate partitioning, endocytosis, protoplasts, sucrose transport, sucrose uptake, vacuole |
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