Baculovirus defective interfering particles are responsible for variations in recombinant protein production as a function of multiplicity of infection |
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| Authors: | T. J. Wickham T. Davis R. R. Granados D. A. Hammer M. L. Shuler H. A. Wood |
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| Affiliation: | (1) School of Chemical Engineering, Cornell University, Olin Hall, 14853 Ithaca, NY;(2) Boyce-Thompson Institute for Plant Research, Tower Road, 14853 Ithaca, NY |
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| Abstract: | Summary Defective interfering particles, present within a high passage inoculum ofAutographa californica nuclear polyhedrosis virus (AcMNPV), interfered with recombinant  -galactosidase and infectious virus production in three insect cell lines. These particles were Selected during serial passage, were missing large parts of the AcMNPV genome, and caused large reductions in -galactosidase production at multiplicities of infection above 0.01 pfu/cell. |
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