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Isopenicillin N epimerase activity in a high cephalosporin-producing strain of Cephalosporium acremonium |
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| Authors: | Claus Lübbe Saul Wolfe Arnold L. Demain |
| Affiliation: | (1) Fermentation Microbiology Laboratory, Department of Applied Biological Sciences, Massachusetts Institute of Technology, 02139 Cambridge, MA, USA;(2) Department of Chemistry, Queens University, K7L 3N6 Kingston, Ontario, Canada;(3) Present address: Fermentation Process Development, Pharmaceutical Research Division, Schering-Plough, 07083 Union, NJ, USA |
| Abstract: | Summary Isopenicillin N epimerase activity in Cephalosporium acremonium CW-19 is so labile that it has never been detected in sonic extracts. Prior to this work, it had only been obtained by the laborious protoplast lysate procedure. The present work shows that the enzyme is present in sonic extracts of a high cephalosporin-producing strain (C-10) of C. acremonium throughout the fermentation. |
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