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Sex chromatin analysis of lymphocytes invading host organs in transfusion-associated graft-versus-host disease |
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| Authors: | H. Matsushita Y. Shibata K. Fuse M. Kimura K. Iinuma |
| Affiliation: | 1. Departments of Pathology, Toranomon Hospital, Toranomon 2-2-2, Minato-Ku, Tokyo 105, Japan 2. Departments of Cardiac Surgery, Toranomon Hospital, Toranomon 2-2-2, Minato-Ku, Tokyo 105, Japan 3. Departments of Immunohematology, Toranomon Hospital, Toranomon 2-2-2, Minato-Ku, Tokyo 105, Japan 4. Departments of Gynecology, Toranomon Hospital, Toranomon 2-2-2, Minato-Ku, Tokyo 105, Japan 5. Division of Clinical Dysmorphology, National Children’s Medical Research Center, Taishidou, 3-35-31, Setagaya-Ku, Tokyo 154, Japan |
| Abstract: | We report a method of sex chromatin analysis of lymphocytes separated from host organs in transfusion-associated graft-versus-host disease (GVHD), which enabled the demonstration of invasion by donor lymphocytes. The lymphocytes examined were separated from deparaffinized tissue blocks of skin, spleen and bone marrow from two female patients with transfusion-associated GVHD by incubation in 0.5% pepsin. The tissues had been removed at autopsy and fixed in formalin. Sex chromatin analysis was performed by fluorescence microscopy on separated lymphocytes stained with 0.005% quinacrine dihydrochloride. By this means Y chromatin-positive (i.e. male) lymphocytes were demonstrated in the skin, spleen and bone marrow of both female patients. |
| Keywords: | Blood transfusion Graft-versus-host disease Lymphocyte Sex chromatin |
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