VEGF concentration from plasma-activated platelets rich correlates with microvascular density and grading in canine mast cell tumour spontaneous model |
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Authors: | Patruno R Arpaia N Gadaleta C D Passantino L Zizzo N Misino A Lucarelli N M Catino A Valerio P Ribatti D Ranieri G |
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Affiliation: | Department of Animal Health and Well-Being, University of Bari Veterinary Medical School, Bari, Italy;Unit of Dermatology, Department of Internal Medicine, Immunology, and Infectious Diseases, Policlinico, University of Bari, Bari, Italy;Unit of Interventional Radiology, Department of Critical Area and Surgery, Istituto Tumori Giovanni Paolo II of Bari, Bari, Italy;Unit of Medical Oncology, Department of Medical Oncology, Istituto Tumori Giovanni Paolo II of Bari, Bari, Italy;Department of Surgery, Ospedale Di Venere, Bari, Italy;Department of Human Anatomy and Histology, University of Bari Medical School, Bari, Italy;Department of Experimental Oncology, Istituto Tumori Giovanni Paolo II of Bari, Bari, Italy |
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Abstract: | Canine cutaneous mast cell tumour (CMCT) is a common cutaneous tumour in dog, with a higher incidence than in human. CMCT is classified in three subgroups, well and intermediately differentiated (G1 and G2), corresponding to a benign disease, and poorly differentiated (G3), corresponding to a malignant disease, which metastasize to lymph nodes, liver, spleen and bone marrow. In this study, we have evaluated serum (S), platelet-poor plasma (P-PP), plasma-activated platelet rich (P-APR) and cytosol vascular endothelial growth factor (VEGF) concentrations, microvascular density (MVD) and mast cell density (MCD) in a series of 86 CMCTs and we have correlated these parameters with each other, by means of ELISA detection of VEGF and immunohistochemistry. Results show that VEGF level from cytosol P-APR and MVD were significantly higher in G3 CMCTs as compared to G1 or G2 subgroups. Moreover, a significantly strong correlation among VEGF levels from P-PAR and cytosol, MVD and MCD was found in G3 subgroup. Because VEGF levels from P-APR well correlated with MVD and malignancy grade in CMCT, we suggest that VEGF might be secreted from MCs and it may be a suitable surrogate inter-species angiogenetic markers of tumour progression in CMCT. Finally, CMCT seems to be a useful model to study the role of MCs in tumour angiogenesis and inhibition of MCs degranulation or activation might be a new anti-angiogenic strategy worthy to further investigations. |
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Keywords: | angiogenesis canine mast cell tumour mast cells microvascular density plasma-activated platelets rich vascular endothelial growth factor |
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