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Light-Induced De-Epoxidation of Violaxanthin in Guard Cell Protoplasts of Vicia faba
Authors:Masamoto  Kazumori; Kinoshita  Toshinori; Shimazaki  Ken-ichiro
Institution:1Biological Laboratory, Faculty of Education, Kumamoto University Kumamoto, 860 Japan
2Department of Biology, Faculty of Science, Kyushu University Hakozaki, Fukuoka, 812 Japan
3Biological Laboratory, College of General Education, Kyushu University Fukuoka, 810 Japan
Abstract:Photosynthetic pigments of Vicia guard cell protoplasts (GCPs)from abaxial epidermis were analyzed by reverse-phase HPLC.Violaxanthin decreased and zeaxanthin increased in GCPs afterlight illumination. The epoxidation state of GCPs decreasedfrom 0.82 (dark) to 0.37 (light), suggesting operation of thexanthophyll cycle in GCPs of Vicia faba. (Received March 15, 1993; Accepted May 10, 1993)
Keywords:
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