The conversion of L-lysine to saccharopine and alpha-aminoadipate in mouse |
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Authors: | K Higashino M Fujioka Y Yamamura |
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Affiliation: | 1. Third Department of Internal Medicine, Osaka University School of Medicine, Osaka 553 Japan;2. College of Bio-Medical Technology, Osaka University, Toyonaka, Osaka 560, Japan |
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Abstract: | Saccharopine [?-N-(l-glutaryl-2)-l-lysine] has been found to occur in normal, untreated mouse liver. The pool of saccharopine as well as that of α-aminoadipate become labeled shortly after the administration of l-lysine-U-14C into intact mouse. In vitro experiments using the mouse liver homogenate have shown that l-lysine is converted to saccharopine in the presence of α-ketoglutarate and NADPH, and saccharopine to α-aminoadipate in the presence of NAD+. The oxidation of α-aminoadipic-δ-semialdehyde (Δ1-piperideine-6-carboxylate), the proposed reaction product of saccharopine cleavage, to α-aminoadipate is effected by either NAD+ or NADP+. |
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