Effects of actinomycin D, amethopterin, and 5-fluro-2'-deoxyuridine on procentriole formation in Chinese hamster fibroblasts in culture |
| |
Authors: | P H DeFoor E Stubblefield |
| |
Institution: | The University of Texas M. D. Anderson Hospital and Tumor Institute, and the Graduate School of Biomedical Sciences, Houston, Tex. 77025, USA |
| |
Abstract: | Synchronized Chinese hamster fibroblasts (Don-C) were cultured for 6 h in the presence of amethopterin, 5-fluorodeoxyuridine (FUdR), or actinomycin D and were then examined by electron microscopy of thin sections for the presence or absence of daughter procentrioles. Control cells were at mid S phase and contained obvious procentrioles in about 1 profile out of 140. Treatment with FUdR or amethopterin did not reduce this frequency significantly. However, serial section analysis did demonstrate a lack of procentrioles in some cells treated with amethopterin. Actinomycin was quite effective in preventing daughter procentriole formation. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|