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Regulation of the in vitro synthesis of E. coli ribosomal protein L12.
Authors:G Goldberg  T Zarucki-Schulz  P Caldwell  H Weissbach  N Brot
Institution:Roche Institute of Molecular Biology, Nutley, New Jersey 07110 USA
Abstract:The invitro DNA dependent synthesis of ribosomal protein L12 and the β subunit of RNA polymerase has been investigated using DNA from a plasmid which contains the genetic information for ribosomal protein L12 and the β subunit of RNA polymerase. This DNA, however, lacks the promoter region and the genetic information for the first 26 amino acids of ribosomal protein L10. It was found that L12 and the β subunit of RNA polymerase are efficiently synthesized invitro from this DNA. These results suggest that L12 and the β subunit of RNA polymerase can be synthesized from a promoter situated within the L10 gene.
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