石油集输系统中微生物群落结构研究

采用16SrRNA基因克隆一变性梯度凝胶电泳分析方法研究了石油集输系统原油和油田产水中的微生物群落结构。变性梯度凝胶电泳图谱显示：油田产水中微生物群落远比原油中的菌群丰富。所有的油田水样和原油样本中都存在与Ochrobactrum sp．和Stenotrophomonas sp．相关的细菌；原油样本中检测出与Burkholderia sp．、Brevundimonas sp．和Propionibacterium sp．相关的细菌，而这些细菌在油田水样中未检出；在油田水样中检出与Hippea sp．、Acidovorax sp．、Arcobacter sp．、Pseudomonas sp．、Thiomicrospira sp．、Brevibacterium sp．、Tissierella sp．和Peptostreptococcus sp．相关的细菌，而这些细菌在原油样本中未检出。用古细菌特异性引物进行检测发现在油田水样中存在与Methanomicrobials和Methanosarcinales相关的产甲烷菌，而这些细菌在原油样本中未检出。在石油集输过程中，油田水样和原油中微生物群落的相似性分别为83．3％和88．2％，说明微生物群落结构较为稳定。

Bacterial Communities in Crude Oil Gathering and Transferring System

Microbial community's structure of crude oil and oil field production water from an oil gathering and transferring system were studied adopting 16S rRNA gene cloning-denaturing gradient gel electrophoresis (DGGE) analysis. The atlas of DGGE showed that microbial communities in oilfield production water were far more richer than those in crude oil. All samples from oilfield production water and crude oil existed bacteria related to Ochrobacterium sp. and Stenotrophomonas sp. While samples from crude oil bacteria related to Burkholderia sp., Brevundimonas sp. and Propionibacterium sp. were detected, but none from oilfield production water samples. Bacteria related to Hippea sp., Acidovorax sp., Arcobacter sp., Pseudomonas sp., Thiomicrospira sp., Brevibacterium sp., Tissierella sp. and Peptostreptococcus sp. were detected from oilfield water samples, but none from crude oil samples. It were found Methanomicrobials and Methanosarcinales methane-producing related bacteria existed in oilfield water samples in the determination using paleo-bacterial specific primers, but none from crude oil samples. The similarity of microbial communities of oilfield water and crude oil samples was 83.3% and 88.2% respectively in the process of gathering and transferring suggested that the microbial community structure was fairly stable.