头孢菌素C产生菌的诱变育种及培养基优化

通过对顶头孢霉（Cephalosporium acremonium）FC-01进行诱变选育及特定种子培养基的优化，提高了头孢菌素C的发酵产量。分别采用紫外-氯化锂和钴-60（60Co）γ射线对FC-01进行诱变选育，筛选到高产菌株FC-1-4和FC-4-2，产量较出发菌株分别提高了26%和54.5%。运用Plackett-Burman设计方法和响应面法对种子培养基进行优化，头孢菌素C发酵效价较对照分别提高了34.7%和13.2%，优化后的种子培养基主要成分为玉米浆3.70%、葡萄糖2.62%和硫酸镁0.15%，得到的菌株及相应的种子培养条件已成功应用在160M³工业发酵罐生产中，具有重要的工业生产能力。

Mutagenesis Breeding and Optimization of Cephalosporin C by Cephalosporium acremonium

The Cephalosporin C fermentation was improved through mutations and optimization of the seed medium. High-producing strains Cephalosporium acremonium FC-1-4 and FC-4-2 were obtained with ⁶⁰Co irradiation and Uv+LiCl treatment, the production were 26% and 54.5% higher than that of control. Meanwhile, the optimal seed medium component were 2.62% glucose, 3.70% corn steep liquor, 0.15% MgSO₄ via Plackett-Burman and central composite design(CCD). The 34.7% and 13.2% increase of Cephalosporin C production was achieved with the Cephalosporium acremonium FC-1-4 and FC-4-2 respectively. The obtained high-producing strains and optimal seed medium have crucial industrial utilization in the future.