基因捕获技术的现状及应用

随着越来越多生物基因组测序的完成,生物医学研究已进入后基因组时代。基因捕获技术由于其在克隆、发现新基因及揭示基因功能方面具有独特的优点,已成为功能基因组时代研究的有力工具,在生物医学研究各个领域的应用日趋广泛。主要对基因捕获技术的原理、分类、一般操作流程、优缺点及基因捕获在发育生物学、新基因鉴定、干细胞分化、肿瘤和生殖医学研究方面的应用作一综述,为相关研究者提供借鉴。

Current Status and Application of Gene Trapping

With the completion of genome sequencing on more and more organisms, post-genomic era has begun. Gene trapping, as a powerful tool in the functional-genomic era, has been widespreadly applicated in many fields of biomedicine research because of its great merits in cloning, finding novel genes and unraveling their biological functions. The principles, classifications, manipulation procedures, advantages and disadvantages of gene trap technique and its application in developmental biology, novel gene identification, stem cells differentiation, cancer and reproductive medicine research are discussed, which will provide helpful references for relevant researchers.