Flow in the well: computational fluid dynamics is essential in flow chamber construction |
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Authors: | Markus Vogel Jörg Franke Wolfram Frank Horst Schroten |
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Institution: | 1.Pädiatrische Infektiologie, Klinik für Allgemeine Pädiatrie, Universitätsklinikum Düsseldorf, Moorenstr. 5, 40225 Düsseldorf, Germany ;2.Institut für Fluid- und Thermodynamik, Universität Siegen, Paul-Bonatz Str. 9-11, 57076 Siegen, Germany |
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Abstract: | A perfusion system was developed to generate well defined flow conditions within a well of a standard multidish. Human vein
endothelial cells were cultured under flow conditions and cell response was analyzed by microscopy. Endothelial cells became
elongated and spindle shaped. As demonstrated by computational fluid dynamics (CFD), cells were cultured under well defined
but time varying shear stress conditions. A damper system was introduced which reduced pulsatile flow when using volumetric
pumps. The flow and the wall shear stress distribution were analyzed by CFD for the steady and unsteady flow field. Usage
of the volumetric pump caused variations of the wall shear stresses despite the controlled fluid environment and introduction
of a damper system. Therefore the use of CFD analysis and experimental validation is critical in developing flow chambers
and studying cell response to shear stress. The system presented gives an effortless flow chamber setup within a 6-well standard
multidish. |
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Keywords: | Cell culture Computational fluid dynamics Endothelium Venous macrovascular Parallel plate flow chamber Shear force |
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