Construction of an ordered cosmid collection of the Escherichia coli K-12 W3110 chromosome. |
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| Authors: | S Tabata A Higashitani M Takanami K Akiyama Y Kohara Y Nishimura A Nishimura S Yasuda Y Hirota |
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| Affiliation: | Department of Biology, Faculty of Science, Nagoya University, Japan. |
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| Abstract: | A cosmid library of the Escherichia coli K-12 W3110 chromosome was constructed in which clones were assigned to locations on the chromosome map by hybridization and genetic marker complementation tests. Approximately 70% of the genome was represented by this library. The identified clones can be maintained in the homologous system and would facilitate genetic studies of E. coli. |
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