The cloning of the rorB gene of Escherichia coli |
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| Authors: | Paul G. Debenham Michael B. T. Webb John Law |
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| Affiliation: | (1) Division of Cell and Molecular Biology, M.R.C. Radiobiology Unit, Chilton, Didcot, Oxon, UK;(2) Present address: Cellmark Diagnostics, Abingdon, Oxon, UK |
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| Abstract: | Summary A segment of the Escherichia coli genome which complements the ionising radiation sensitivity of the rorB mutation was cloned into pBR322. This DNA segment also complements the mitomycin C sensitivity of the rorB mutation. The gene was subcloned until defined in a fragment of 1.05 kb. Only one gene product, a protein of approximately 16.5 kDa, was found on maxicell analysis of the various subclones. Iso-electric focusing of this gene product suggests it may function in a complex. |
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| Keywords: | Ionising radiation Gene cloning DNA repair Escherichia coli Mitomycin C |
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