Changes in Endogenous Gibberellin Contents during Growth of Cultured Tobacco Cells |
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Authors: | Park Keun-Hyung; Fujisawa Sachiko; Sakurai Akira; Yamaguchi Isomaro; Takahashi Nobutaka |
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Institution: | 1The Institute of Physical and Chemical Research Wako-shi, Saitama 351, Japan
2Department of Agricultural Chemistry, The University of Tokyo Bunkyo-ku, Tokyo 113, Japan |
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Abstract: | Changes in the contents of endogenous gibberellins (GAs) wereexamined in three kinds of cultured tobacco cells; a crown gallcell and two cultured cells derived from normal tissue of Nicotianatabacum. The relative amounts of the GAs were analyzed by systematicchromatographic purifications followed by GC-SIM. In all thecell lines examined, the content of GAj was the highest duringthe logarithmic phase of growth, indicating that GA1 has a physiologicalrole in the growth of dedifferentiated cells.
3 Present address: College of Agriculture, Chonnam NationalUniversity, Kwangju 500, Korea. (Received April 11, 1984; Accepted July 10, 1984) |
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