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Changes in Endogenous Gibberellin Contents during Growth of Cultured Tobacco Cells
Authors:Park  Keun-Hyung; Fujisawa  Sachiko; Sakurai  Akira; Yamaguchi  Isomaro; Takahashi  Nobutaka
Institution:1The Institute of Physical and Chemical Research Wako-shi, Saitama 351, Japan
2Department of Agricultural Chemistry, The University of Tokyo Bunkyo-ku, Tokyo 113, Japan
Abstract:Changes in the contents of endogenous gibberellins (GAs) wereexamined in three kinds of cultured tobacco cells; a crown gallcell and two cultured cells derived from normal tissue of Nicotianatabacum. The relative amounts of the GAs were analyzed by systematicchromatographic purifications followed by GC-SIM. In all thecell lines examined, the content of GAj was the highest duringthe logarithmic phase of growth, indicating that GA1 has a physiologicalrole in the growth of dedifferentiated cells. 3 Present address: College of Agriculture, Chonnam NationalUniversity, Kwangju 500, Korea. (Received April 11, 1984; Accepted July 10, 1984)
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