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Detection of double-stranded RNA molecules and virus-like particles in different Mucor species
Authors:Csaba Vágvölgyi  Klaudia Magyar  Tamás Papp  Mária Vastag  Lajos Ferenczy  László Hornok  Csaba Fekete
Institution:(1) Department of Microbiology, Attila József University, P.O. Box 533, H-6701 Szeged, Hungary;(2) Department of Microbiology, Agricultural University, Károly Páter 1, H-2103 Gödöllõ, Hungary;(3) Agricultural Biotechnology Center, Institute of Plant Sciences, P.O. Box 411, H-2101 Gödöllõ, Hungary
Abstract:The presence of double-stranded RNA elements was examined in 123 strains representing 18 Mucor species. These genetic elements were found to be present in 6 strains: 1 M. aligarensis, 1 M. hiemalis, 2 M. corticolus, 1 M. mucedo and 1 M. ramannianus. Electrophoretic separation of the nucleic acids revealed 4 different RNA patterns, with 1 to 5 discrete dsRNA bands. The molecular weights corresponding to these bands were 1.42–4.15 × 106 D. Using electronmicroscopy, for the first time the presence of virus like particles in Mucor species has been revealed.
Keywords:DsRNA  Mucor  Mycovirus  VLP
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