A molecular genetic linkage map of mouse chromosome 18, includingspm,Grl-1, Fim-2/c-fms,andMbp |
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| Authors: | Yuka Sakai Shigeki Miyawaki Atsuko Shimizu Kyoko Ohno Tomomasa Watanabe |
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| Institution: | (1) Department of Biochemistry, Institute for Developmental Research, Aichi Prefecture Colony, Kamiyacho, 480-03 Kasugai, Aichi, Japan;(2) Research Laboratories, Nippon Shinyaku Co., Sakanotsujicho, Oyake, 607 Yamashinaku, Kyoto, Japan;(3) Laboratory of Experimental Animals, Yagi Memorial Park, 505-01 Mitake, Gifu, Japan |
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| Abstract: | Restriction endonuclease fragment length variations (RFLV) were detected in mice with DNA probes for myelin basic protein (Mbp), glucocorticoid receptor-1 (Grl-1), and Friend MuLV integration site-2 (Fim-2). RFLV of theMbp gene were found inSacI restriction patterns, RFLV of theGrl-1 gene were found inEcoRV patterns, and RFLV of theFim-2 were found inBglII patterns. A three-point backcross was carried out by the backcross mating (C57BL/KsJ-spm/spm × MOL-MIT)F1 males × C57BL/KsJ-spm/spm; spm is an autosomal recessive gene causing sphingomyelinosis. From the results,spm, Grl-1, Fim-2, andMbp loci were mapped on chromosome 18, and the following order of genes is proposed, with distances between genes in parentheses: centromere—spm—(7.8 cM)—Grl-1—(7.8 cM)—Fim-2—(39.1 cM)—Mbp—telomere. All laboratory strains and two European subspecies (Mus mus domesticus andM. m. brevirostris) carry theGrl-1
a
,Fim-2
a
, andMbp
a
alleles. In contrast, another wild subspecies from Europe (M. m. musculus) and some Asian subspecies (M. m. molossinus, Chinese mice of wild origin, andM. m. yamashinai) carry theGrl-1
b
,Fim-2
b
, andMbp
b
alleles. Onlycastaneus strains carry the intermediate combination of theGrl-1
b
,Fim-2
a
, andMbp
b
alleles. |
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| Keywords: | mice subspecies sphingomyelinosis glucocorticoid receptor Friend MuLV integration site myelin basic protein linkage test restriction fragment length variations (RFLV) |
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