Apyrene sperm from the triploid donors restore fecundity of cryopreserved semen in Bombyx mori |
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Authors: | Takemura Yoko Sahara Ken Mochida Yuji Ohnuma Akio |
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Affiliation: | a Institute of Sericulture, 1053 Iikura, Ami-machi, Ibaraki 300-0324, Japan b Laboratory of Applied Molecular Entomology, Graduate School of Agriculture, Hokkaido University, Sapporo 060-8589, Japan |
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Abstract: | Female moths of Bombyx mori were artificially inseminated with cryopreserved semen. The fertility of inseminated females varied from 0% to 76.9% depending on the strain. Addition of fresh semen from triploid males, which are infertile but whose semen includes intact apyrene sperm, greatly improved fecundity of cryopreserved semen from normal males. Frozen apyrene sperm from the triploid donors also improved the fecundity of females, inseminated with cryopreserved normal semen, but less than fresh semen from triploid males. Fertilization success in B. mori requires the presence of both, intact eupyrene and apyrene sperm. Our results show that eupyrene sperm tolerate the cryopreservation process better than apyrene sperm. Hence, we recommend to add apyrene sperm from the triploid donors as helper sperm routinely to cryopreserved semen in artificial insemination. This may advance the application of cryopreservation as a routine technique to maintain silkworm resources. The technique may also be applicable to other moth and butterfly species which, like B. mori, possess eupyrene and apyrene sperm. |
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Keywords: | Apyrene sperm Artificial insemination Cryopreservation Eupyrene sperm Silkworm |
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